The Famous Coulter Principle
Coulter principle meanings that: the molecule suspended in the electrolyte with the electrolytic method thru the orifice tube, the persistent current circuit style results in holes between the inside and outside tubes an instantaneous changes in the resistance between two electrodes, a potential pulse. Size and number and size of the particle are proportional to the number of pulse signals. This is primarily dependent on the blood cells as compared to diluent, poor blood cells are created by the characteristics of the conductor. Originally, just the original calculation and measurement Coulter counter erythrocytes. Later on, with the constant improvement of technology keeps to evolve and equipment, physicians also can use it to estimate and evaluate the white blood cells. Up to the 1970s, the further development of technology enables experts to separate platelets.
Development of automatic cell counters
Cytometer in the conventional sense is simply by studying the peripheral blood smear, making use of blood cells and white blood cell count and device done by hand (also identified as 100 smear classification, manual leukocyte count or manual counters). Based upon Kurt principle contributed to the invention of the Coulter counter, and consequently developed a technologically advanced automated hematology analyzer. Since that time, the level of instrument’s technical has been increasing. As a consequence of improves in tech, a single instrument might be analyzed more details, so significantly increasing the efficacy of the blood testing, the decrease of a multiple trial analysis instrument situation. Present cell analyzer capable of calculating white blood cell (wbc), leukocyte(five categories), hemoglobin (hgb), platelets (plt), red blood cells (rbc), mean corpuscular volume (mcv), mean platelet volume, which can automatically calculate blood cells hematocrit (hct), mean corpuscular hemoglobin concentration (mchc), mean corpuscular hemoglobin (mch), red blood cell distribution width, platelet volume and platelet distribution width.
Other crucial aspects consist of automated blood analyzer speed and they run the amount of samples that can be handled per batch (large processing capacity can reduce the turnaround time).
The Instant test (poct)
Instant test (poct) is being an worthwhile associate to the blood lab practice. WBC count is often a blood cell analyzer has been in the unit of intensive care and other places have been used practically. Compare hands-on cell counting and automated cell count
Even though the peripheral blood smear evaluation of the important information supplied is automated cell count cannot be gained, but it still has some restrictions and some unique demands.
Limitations of Manual cell count
Will need to have some undergo to be able to make smears technically preserve sufficient standardization and uniformity in the operation.
-Is very subjective, labor-extensive and statistically untrustworthy (calculated only 100-200 cells).
-Coefficient of variance stated (cv) from 30% to 110 %, and is incorrect.
-checking the cells manually identification mistake: This is commonly lymphocytes and monocytes want with cell division and unnatural types of variations (lymphocytes and blasts) separate. Monocytes are often underrated, and lymphocytes are often overestimated.
The Automatic Analyzer’s Advantage
Making use of an automated biochemistry and chemistry analyzer for sale cell count is a rapidly, unbiased, statistically important (calculated over 8000 cells), and may not be counted manually caused by various biases impacted. In contrast to methods of manual, automatic analyzer is more reliable and more affordable. Various automated analyzer can deal with 120-150 samples every hour. Furthermore, to make sure precise automatic category of white blood cell count complete stability and reproducibility.
Here the Automatic Hematology Analyzer MSLAB07
Drawbacks of automated analyzer
Automated blood cell analyzer might also result cell count far too much or weak hands of the phenomenon. Many analyzers particularly impedance form counter only tests the size and number of particles, might not properly separate tiny platelet aggregation and nucleated red blood cells. Platelet aggregation can be inaccurately categorized as white blood cells or red blood cells, and nucleated red blood cells might also be mistakenly classified as white blood cells, lymphocytes are classified as very vulnerable. What’s more, large or illegible irregular cells, immature neutrophils and harmful sensitive lymphocytes are also apt to be improperly classified.